Tuesday, 03/26/2024
2:00 PM
Steinman Hall #312
Professor Prajna Dhar
University of Kansas
Department of Chemical and Petroleum Engineering
“Interface Induced Aggregation and Protein Particle Formation in Antibody-based Therapeutic Formulations”
ABSTRACT
The air-liquid interface has been recognized to play a significant role in protein stability throughout bioprocessing and manufacturing, storage, and even clinical use. As proteins adsorb to the air-liquid interface, they tend to unfold and potentially form aggregates at the interface. These aggregates can be lost to the bulk solution, due to presence of stresses acting at the interface. In this talk, I will present various interfacial techniques to corelate the interfacial properties of the adsorbed protein films (under static and applied stress conditions) with protein particle formation at the interface and in the bulk solution. We will also discuss surfactant-based mitigation strategies and the role of competitive adsorption in preventing interface-induced protein particle formation.
MOST RECENT RESEARCH INTERESTS:
Protein stability at interfaces, lipid/protein interactions (particularly in disease progression), surfactant degradation and its impact on protein stability in therapeutic formulations, interfacial rheology.
